VASP anticorps (pSer239) (Biotin)

N° du produit ABIN126909

Cet anticorps anti-VASP est un anticorps Souris Monoclonal détectant VASP dans WB, IF, IP, FACS et EIA. Adapté pour Humain et Souris.

Aperçu rapide pour VASP anticorps (pSer239) (Biotin) (ABIN126909)

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Reactivité: Humain, Souris

Hôte: Souris

Clonalité: Monoclonal

Conjugué: Cet anticorp VASP est conjugé à/à la Biotin

Application: Western Blotting (WB), Immunofluorescence (IF), Immunoprecipitation (IP), Flow Cytometry (FACS), Enzyme Immunoassay (EIA)

Clone: 16C2

Détail du produit anti-VASP anticorps (Biotin)

Épitope: pSer239

Specificité: This antibody recognizes VASP only, when Ser 239 is phosphorylated, a site preferred by cGMP-dependent protein kinase (PKG) but also used by cAMP-dependent protein kinase (PKA). The antibody does not crossreact with the non-phosphorylated form of VASP nor with unrelated serine-phosphorylated proteins. Therefore, antibody VASP-16C2 is able to monitor the phosphorylation state of VASP serine 239 as well as PKA activity.

Attributs du produit: Synonyms: Vasodilator-stimulated phosphoprotein

Purification: Size exclusion chromatography

Immunogène: Synthetic phosphopeptide conjugated to KLH

Isotype: IgG1

Information d'application

Indications d'application: Western Blot: 0.5 μg/mL for HRPO/ECL detection. Recommended blocking buffer: BSA/Tween 20 based blocking and blot incubation buffer. ELISA: 0.05 μg/mL. Immunoprecipitation: 1 - 10 g per 106 pervanadate-treated A431 cells. Immunocytochemistry: 1 - 10 μg/mL. Mab VASP-16C2 may tolerate 0.5 % formaldehydefixation. Flow Cytometry.
Other applications not tested.
Optimal dilutions are dependent on conditions and should be determined by the user.

Restrictions: For Research Use only

Stockage

Concentration: 0.5 mg/mL

Buffer: 2 x PBS with 0.09 % Sodium Azide, PEG and Sucrose

Agent conservateur: Sodium azide

Précaution d'utilisation: This product contains sodium azide: a POISONOUS AND HAZARDOUS SUBSTANCE which should be handled by trained staff only.

Stock: 4 °C/-20 °C

Stockage commentaire: Store the antibody (Aliquote and freeze in liquid nitrogen) at -20 °C. Avoid repeated freezing and thawing. Thaw aliquots at 37 °C. Thawed aliquots may be stored at 4 °C up to 3 months.
Shelf life: one year from despatch.

Date de péremption: 12 months

Détails sur VASP

Antigène: VASP (Vasodilator-Stimulated phosphoprotein (VASP))

Autre désignation: VASP

Sujet: VASP (vasodilator stimulated phosphoprotein) plays an important role in ANF / NO / cGMP Protein kinase and cAMP / cAMP Protein kinase signalling pathways. VASP is expressed in almost all human and animal cell lines, particularly high concentrations are found in thrombocytes, vascular smooth muscle cells and fibroblasts. In cultured cells VASP is associated with focal contacts, cell-cell-contacts, microfilaments and dynamic membrane regions such as the leading edge. In vitro binding data show that VASP binds to profilin, zyxin, vinculin, and the Listeria spp. surface protein ActA. Functional evidence indicates that VASP is a crucial factor involved in the enhancement of actin filament formation.Synonyms: Vasodilator-stimulated phosphoprotein

ID gène: 7408

UniProt: P50552

Pathways: TCR Signaling, Regulation of Actin Filament Polymerization, Tube Formation